Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW264.7)
Loading amount
50000 cells
Specification
RAW264.7
Treatment
100 ng/ml recombinant mouse RANKL
Gel Running Conditions
Reduced Denaturing (NuPage 10% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/700
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 58 kDa
Negative control
Cell lysate NIH-3T3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 01 2010