Anti-TPR 抗体 (ab70610)
Key features and details
- Rabbit polyclonal to TPR
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TPR antibody
TPR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TPR -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residue 1650 and 1700 of human TPR
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70610 was affinity purified using an epitope specific to TPR immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70610の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 267 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 267 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint. -
組織特異性
Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney. -
関連疾患
Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 7175 Human
- Entrez Gene: 108989 Mouse
- Entrez Gene: 304862 Rat
- Omim: 189940 Human
- SwissProt: P12270 Human
- SwissProt: F6ZDS4 Mouse
- SwissProt: F1MA98 Rat
- Unigene: 279640 Human
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別名
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
画像
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All lanes : Anti-TPR antibody (ab70610) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 267 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Additional bands at: 235 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Detection of Human TPR by Western Blot of Immunprecipitate. ab70610 at 1µg/ml staining TPR in HeLa whole cell lysates immunoprecipitated using ab70610 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70610 は 3 報の論文で使用されています。
- Lee ES et al. TPR is required for the efficient nuclear export of mRNAs and lncRNAs from short and intron-poor genes. Nucleic Acids Res 48:11645-11663 (2020). PubMed: 33091126
- López-Soop G et al. AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint. Cell Cycle 16:947-956 (2017). PubMed: 28379780
- Myers KN et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . PubMed: 27739501