Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti goat IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted image of Substantia nigra, I think, shows a distinct pattern of positivity ( upper image) which appears to be complementary to that obtained on near sequential section using anti TH ab737869 ( lower image).
Very fine fibres in frontal cortex and Hippocampus are also positive).
In my hands, this ab is not quite as sensitive ( signal:noise) as ab121020.
However, I am not familiar with the distribution pattern of TPH2.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Sep 26 2014