Application
Western blot
Loading amount
1.2e+006 cells
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - nuclear (Ins-1, a rat insulinoma cell line)
Specification
Ins-1, a rat insulinoma cell line
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Polyclonal Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 75 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Javier Triñanes
Verified customer
投稿 Dec 23 2013