Anti-TOR1AIP1 抗体 (ab86307)
Key features and details
- Rabbit polyclonal to TOR1AIP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TOR1AIP1 antibody
TOR1AIP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TOR1AIP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region between residues 175 and 225 of human TOR1AIP1 (NP_056417.2).
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ポジティブ・コントロール
- WB: Jurkat nuclear extract lysate (ab14844), HeLa and 293T whole cell lysates. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab86307 was affinity purified using an epitope specific to TOR1AIP1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina. -
配列類似性
Belongs to the TOR1AIP family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 26092 Human
- SwissProt: Q5JTV8 Human
- Unigene: 496459 Human
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別名
- Lamin-associated protein 1B antibody
- Lamina associated polypeptide 1B antibody
- Lamina associated polypeptide 1C antibody
see all
画像
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All lanes : Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 30 seconds -
Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab86307 は 1 報の論文で使用されています。
- Luithle N et al. Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1. Elife 9:N/A (2020). PubMed: 33320087