Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
25 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST/5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL
Exposure
16 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 16 kDa Non-specific: 35, 45, 55, 60... kDa
Additional data
Abcam response
Thank you for your honest and valuable feedback. Xenopus laevis is not a tested species for ab78547 and your data suggest it may not be suitable for use in this species under the conditions in which you tested. Many thanks for your feedback on this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 21 2010