Anti-TOM1L2 抗体 (ab121716)
Key features and details
- Rabbit polyclonal to TOM1L2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TOM1L2 antibody
TOM1L2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TOM1L2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
antigen sequence, corresponding to amino acids 158-242 (ALSPIHTPQR SVPEVDPAAT MPRSQSQQRT SAGSYSSPPP APYSAPQAPA LSVTGPITAN SEQIARLRSE LDVVRGNTKV MSEML) of Human TOM1L2.
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ポジティブ・コントロール
- Human heart muscle tissue; RT4, U251 MG, Human plasma, liver and tonsil lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121716の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Probable role in protein transport. May regulate growth factor-induced mitogenic signaling. -
組織特異性
Ubiquitously expressed with higher expression in heart and skeletal muscle. -
配列類似性
Belongs to the TOM1 family.
Contains 1 GAT domain.
Contains 1 VHS domain. -
ドメイン
The GAT domain mediates interaction with TOLLIP. - Information by UniProt
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参照データベース
- Entrez Gene: 146691 Human
- Entrez Gene: 216810 Mouse
- Entrez Gene: 360537 Rat
- Omim: 615519 Human
- SwissProt: Q6ZVM7 Human
- SwissProt: Q5SRX1 Mouse
- Unigene: 462379 Human
- Unigene: 218875 Mouse
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別名
- FLJ32746 antibody
- Srebf1 antibody
- Target of Myb-like protein 2 antibody
see all
画像
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Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121716 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121716 at 1/250 dilution staining TOM1L2 in Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
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All lanes : Anti-TOM1L2 antibody (ab121716) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver tissue lysate
Lane 5 : Human Tonsil tissue lysate
Developed using the ECL technique. -
Immunohistochemical staining of Human heart muscle shows moderate cytoplasmic positivity in myocytes using ab121716
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121716 は論文での使用が確認できていません。