Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Thymus)
Specification
Thymus
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% Milk powder/ PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/50
Additional data
Additional Notes
Endogenous HRP was blocked with 3% H2O2 and 1% NaN3 for 30 minutes before serum block.
Abcam response
IHC-Fr has never been tested
seconday from Jackson
seconday from Jackson
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 17 2009