Application
Western blot
Sample
Human Recombinant protein (Recombinant Human TFN-alpha (Source: E coil))
Loading amount
0.5 µg
Specification
Recombinant Human TFN-alpha (Source: E coil)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/16000
Detection
Detection method
ECL+
Exposure
3 second(s)
Bands
Specific: 17.5 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 15 2010