Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Rabbit Tissue lysate - whole (aortic tissue)
Specification
aortic tissue
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
ECL
Bands
Specific: 17 KDa/26 Kda kDa Non-specific: 50 KDa kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
Liver from rat treated with LPS. We detected bands at the same location (17/26 and 50 KDa)in this sample. No image is available.
Additional data
Additional Notes
According with the literature, we suspect that bands at 50 KDa may be an heterodimer
PubMed ID
23335007: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 09 2013