Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Coronal brain slices)
Specification
Coronal brain slices
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% goat serum in PBS 0.05% triton-x100
Dilution
1/500
Additional data
Additional Notes
Mice were perfused with PBS, and brains were stored in 4% PFA for 24 hr in 4 Celsius and then 30% sucrose for 48 hr 4 Celsius.
Slice thickness: 30um.
Blocking: 20% goat serum for 1.5 hr RT. Co- stained with rabbit anti-GFAP (Sigma 1:400), 2nd antibody: Goat anti-rabbit Alexa 647 Life technologies (1:500). Both secondary antibodies: 2 hrs, diluted in 2% goat serum in PBS 0.05% Triton-X100. Green- TNF reactivity, Purple- GFAP(astrocytes) reactivity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Ronit Shapira
Verified customer
投稿 Mar 23 2016