Anti-TMEM16A 抗体 (ab53212)
Key features and details
- Rabbit polyclonal to TMEM16A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TMEM16A antibody
TMEM16A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TMEM16A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human TMEM16A aa 400-500. The exact sequence is proprietary.
Database link: Q5XXA6 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53212の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (2) |
1/100.
|
特記事項 |
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IHC-P
1/100. |
ターゲット情報
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機能
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
組織特異性
Broadly expressed with higher levels in liver and skeletal muscle. -
配列類似性
Belongs to the anoctamin family. -
ドメイン
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
細胞内局在
Cell membrane. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 55107 Human
- Entrez Gene: 101772 Mouse
- Entrez Gene: 309135 Rat
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- SwissProt: Q8BHY3 Mouse
- Unigene: 503074 Human
- Unigene: 26700 Mouse
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別名
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
画像
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ab53212 (2µg/ml) staining TMEM16A in human liver (left panel) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of hepatocyte cell membrane.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal stromal tumor labeling TMEM16A with ab53212.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (101)
ab53212 は 101 報の論文で使用されています。
- Li XL et al. Blockade of TMEM16A protects against renal fibrosis by reducing intracellular Cl- concentration. Br J Pharmacol 179:3043-3060 (2022). PubMed: 34961937
- Zhang Y et al. TMEM16A Plays an Insignificant Role in Myocardium Remodeling but May Promote Angiogenesis of Heart During Pressure-overload. Front Physiol 13:897619 (2022). PubMed: 35711304
- Su S et al. A Mathematical Model of Salivary Gland Duct Cells. Bull Math Biol 84:84 (2022). PubMed: 35799078
- Kawata N et al. Increased TMEM16A-Mediated Ca2+-Activated Cl- Currents in Portal Vein Smooth Muscle Cells of Caveolin 1-Deficient Mice. Biol Pharm Bull 45:1692-1698 (2022). PubMed: 35989294
- Lin JM et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons. Elife 11:N/A (2022). PubMed: 36111787