Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Mouse Tissue sections (frozen sections from 13.5dpc mouse embryo and plac)
Specification
frozen sections from 13.5dpc mouse embryo and plac
Permeabilization
No
Fixative
Acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% ALBUMIN IN TBS
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: AffiniPure Goat anti-Rabbit (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Ira Rostovsky
Verified customer
投稿 Feb 16 2015