Anti-TLR4 抗体 [HTA125] (ab8376)
Key features and details
- Mouse monoclonal [HTA125] to TLR4
- Suitable for: IP, Flow Cyt
- Reacts with: Rat, Guinea pig, Human, Pig, Rhesus monkey
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-TLR4 antibody [HTA125]
TLR4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HTA125] to TLR4 -
由来種
Mouse -
特異性
This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. We do not guarantee WB since we receive variable results from our customers and cannot make this antibody work in our hands in WB in denaturing and reducing conditions. The immunogen is a full-length immunogen so we suspect that to get the product to work in WB, non-reducing or native WB could be a better option. THP1 monocytes or macrophage are good positive controls and HeLa/Hek293 should be suitable negative/low expression controls. Membrane preparation may be better that whole cell as expression levels may not be that high in some samples. We welcome any further feedback in WB by participating to our AbTrial program. -
アプリケーション
適用あり: IP, Flow Cytmore details -
種交差性
交差種: Rat, Guinea pig, Human, Pig, Rhesus monkey -
免疫原
Tissue, cells or virus corresponding to TLR4. Ba/F3 cell line expressing TLR4
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
HTA125 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
1/10 - 1/25.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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特記事項 |
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IP
Use at an assay dependent concentration. |
Flow Cyt
1/10 - 1/25. Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
組織特異性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
関連疾患
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
ドメイン
The TIR domain mediates interaction with NOX4. -
翻訳後修飾
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7099 Human
- Entrez Gene: 399541 Pig
- Entrez Gene: 29260 Rat
- Omim: 603030 Human
- SwissProt: O00206 Human
- SwissProt: Q68Y56 Pig
- SwissProt: Q9QX05 Rat
- Unigene: 174312 Human
see all -
別名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab8376 は 30 報の論文で使用されています。
- Wardani G et al. Antioxidative Stress and Anti-Inflammatory Activity of Fucoidan Nanoparticles against Nephropathy of Streptozotocin-Induced Diabetes in Rats. Evid Based Complement Alternat Med 2022:3405871 (2022). PubMed: 35685736
- Wang X et al. Danshen (Salvia miltiorrhiza) restricts MD2/TLR4-MyD88 complex formation and signalling in acute myocardial infarction-induced heart failure. J Cell Mol Med 24:10677-10692 (2020). PubMed: 32757377
- Shi L et al. Chitosan oligosaccharide-mediated attenuation of LPS-induced inflammation in IPEC-J2 cells is related to the TLR4/NF-?B signaling pathway. Carbohydr Polym 219:269-279 (2019). PubMed: 31151525
- Kim SY et al. Isorhamnetin alleviates lipopolysaccharide-induced inflammatory responses in BV2 microglia by inactivating NF-?B, blocking the TLR4 pathway and reducing ROS generation. Int J Mol Med 43:682-692 (2019). PubMed: 30483725
- Cebatariuniene A et al. Extracellular Vesicles Suppress Basal and Lipopolysaccharide-Induced NF?B Activity in Human Periodontal Ligament Stem Cells. Stem Cells Dev N/A:N/A (2019). PubMed: 31017040