Anti-TLR4 抗体 [HTA125] - Low endotoxin, Azide free (ab171260)
Key features and details
- Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-TLR4 antibody [HTA125] - Low endotoxin, Azide free
TLR4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HTA125] to TLR4 - Low endotoxin, Azide free -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ・コントロール
- Human peripheral blood leukocytes
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特記事項
ab171260 has been reported for use in flow cytometric analysis. It has also been reported in blocking of LPS-induced cytokine production. For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti-mouse IgG and streptavidin-PE.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
HTA125 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171260の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 105-8 cells.
A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol rather than Ficoll-gradient prepared PBMCs. The use of a density gradient appears to reduce the staining intensity significantly. |
特記事項 |
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Flow Cyt
Use 1µg for 105-8 cells. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol rather than Ficoll-gradient prepared PBMCs. The use of a density gradient appears to reduce the staining intensity significantly. |
ターゲット情報
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機能
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. -
組織特異性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. -
関連疾患
Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 18 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
ドメイン
The TIR domain mediates interaction with NOX4. -
翻訳後修飾
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7099 Human
- Omim: 603030 Human
- SwissProt: O00206 Human
- Unigene: 174312 Human
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別名
- ARMD10 antibody
- CD284 antibody
- CD284 antigen antibody
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画像
データシートおよび資料
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Datasheet download
参考文献 (3)
ab171260 は 3 報の論文で使用されています。
- Bar-Yoseph H et al. Infliximab-Tumor Necrosis Factor Complexes Elicit Formation of Anti-Drug Antibodies. Gastroenterology 157:1338-1351.e8 (2019). PubMed: 31401142
- Hiraku Y et al. Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation. Part Fibre Toxicol 13:16 (2016). PubMed: 27026438
- Yonekawa K et al. Myeloid related proteins activate Toll-like receptor 4 in human acute coronary syndromes. Atherosclerosis 218:486-92 (2011). PubMed: 21782178