Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Uterus
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Positive control (lymph node) didn't stain, while the smooth muscle portion of the uterus stained, whereas the endometrium didn't.
Abcam response
Thank you for participating in our AbTrial testing program. We have validated this antibody in monkey samples and will continue to gather data on use in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 31 2017