Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (Macrophages)
Specification
Macrophages
Permeabilization
Yes - Triton X 0.1% 5 min
Fixative
Formaldehyde
Other product details
Concentration
0.75 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1%
Secondary antibody
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed
Dilution
1/200
Additional data
Additional Notes
The image below contains, the Flow cytometry performed to confirm if our cell line expresses TLR3. the results were positive (as we kmow it before to buy it).
There were taken images but was imposible to find the TLR3 inside the cells following the protocol above.
Abcam response
Thank you for your feedback. We are sorry to hear that this antibody has not given you good results in immunocytochemistry with murine cells.
Unfortunately we had not previously tested this application in our lab, and it may well be this antibody is not suitable for it. We would welcome more feedback from our customers in order to make any further decision about the application’s suitability.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 23 2014