Anti-TIMP4 抗体 (ab58425)
Key features and details
- Rabbit polyclonal to TIMP4
- Suitable for: ELISA, IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TIMP4 antibody
TIMP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TIMP4 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-Fr, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide derived from C terminal residues of human TIMP4
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at -20°C. Stable for 12 months at -20°C -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 23525608
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 23525608 |
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. -
組織特異性
Abundant in heart and present at low levels in many other tissues. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7079 Human
- Entrez Gene: 110595 Mouse
- Entrez Gene: 680130 Rat
- Omim: 601915 Human
- SwissProt: Q99727 Human
- SwissProt: Q9JHB3 Mouse
- SwissProt: P81556 Rat
- Unigene: 591665 Human
see all -
別名
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
画像
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All lanes : Anti-TIMP4 antibody (ab58425) at 1/500 dilution
Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 cell extract with immunising peptide
Predicted band size: 26 kDa
Observed band size: 26 kDa -
ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab58425 は 13 報の論文で使用されています。
- Chan BYH et al. MMP inhibitors attenuate doxorubicin cardiotoxicity by preventing intracellular and extracellular matrix remodelling. Cardiovasc Res 117:188-200 (2021). PubMed: 31995179
- Dahal S et al. Quantitative Morphometry of Elastic Fibers in Pelvic Organ Prolapse. Ann Biomed Eng 49:1909-1922 (2021). PubMed: 33768411
- Jameson SA et al. Elastin homeostasis is altered with pelvic organ prolapse in cultures of vaginal cells from a lysyl oxidase-like 1 knockout mouse model. Physiol Rep 8:e14436 (2020). PubMed: 32533648
- Jana S et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). PubMed: 30371322
- El Hajj EC et al. Inhibitor of lysyl oxidase improves cardiac function and the collagen/MMP profile in response to volume overload. Am J Physiol Heart Circ Physiol 315:H463-H473 (2018). PubMed: 29775412