Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton X-100/PBS
Specification
HeLa
Blocking step
0.5% BSA, 0.2% fish skin gelatin/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti-goat IgG Alexa 488
Host species: Sheep
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Sheep
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for your honest and valuable feedback. Abcam has not tested ab23938 in ICC/IF and your data suggest it may not be suitable for this application under the conditions in which you tested. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 28 2010