Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Permeabilization
Yes - citrate buffer
Specification
embryo
Blocking step
NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/75
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: alexa fluor 546 donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Additional data
Additional Notes
I also tried without antigen retrieval which also did not stain. If you stain on an unfixed tissue, the antibody stains the heart myocardium.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse or with frozen tissue and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species/application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 29 2016