Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/HCl
Sample
Mouse Tissue sections (melanoma tumour from C57 mice)
Specification
melanoma tumour from C57 mice
Permeabilization
Yes - Tween 20 0,5%
Fixative
Paraformaldehyde
Other product details
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA 0,5% tween 20 1% Donkey serum
Dilution
1/25
Secondary antibody
Name
Non-Abcam antibody was used: AF555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Javier Rodriguez
Verified customer
投稿 Nov 14 2013