Anti-THOC5 抗体 [EPR6905(B)] (ab124996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6905(B)] to THOC5
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-THOC5 antibody [EPR6905(B)]
THOC5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6905(B)] to THOC5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human THOC5 aa 650-750. The exact sequence is proprietary.
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ポジティブ・コントロール
- 293T, HeLa, NIH 3T3 and A549 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6905(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
ターゲット情報
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機能
Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the THOC5 family. -
翻訳後修飾
Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8563 Human
- Entrez Gene: 107829 Mouse
- Entrez Gene: 360972 Rat
- Omim: 612733 Human
- SwissProt: Q13769 Human
- SwissProt: Q8BKT7 Mouse
- SwissProt: Q68FX7 Rat
- Unigene: 75361 Human
see all -
別名
- C22orf19 antibody
- Chromosome 22 open reading frame 19 antibody
- Fmip antibody
see all
画像
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All lanes : Anti-THOC5 antibody [EPR6905(B)] (ab124996) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 79 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab124996 は論文での使用が確認できていません。