Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lung tissue)
Specification
Lung tissue
Fixative
HOPE
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Zytomed Systems Antibody Diluent
Secondary antibody
Name
Non-Abcam antibody was used: One Step Polymer System (Zytomed Systems)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
TGFßRII targeted in alveolar macrophages (A) and lung carcinoma (B).Positive signals indicated by red colour (permanent AEC).
Abcam response
HOPE = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 02 2011