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Products:Signal Transduction >> Growth Factors/Hormones >> TGF
Overview
Product name
TGF beta 2 protein
TGF beta 2 抗体 (2件) 一覧
Protein description
Recombinant full length protein (Human) expressed using (BTI-Tn-5B1-4) cells
Properties
Purity
>95% by SDS-PAGE
Purification notes
Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Form
Lyophilised:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Additional notes
The ED50, determined by hTGF-b2 ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is < 0.2ng/ml, corresponding to a specific activity of > 5 x 106 units/mg.
Expression System = Baculovirus infected High-5 cells.
Sequence notes
hTGF-b2 (Human Transformi ng Growth Factor beta 2) is a 25.0 kDa protein with each subunit containing 112 amino acid residues: ALDAAYC FRN VQDNCCLRP L YIDFKRDL GW KWIHEPK GYN ANFCAG ACPY LWSSD TQHSRVLSLY NTINP EASA SPCCVS QDL EPLTILY YI GKTPKIEQ L SNMIVKSCK CS
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Involvement in disease
Note=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
Sequence similarities
Belongs to the TGF-beta family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP61812
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for TGF beta 2 protein (ab629)
This product has been referenced in:
- Titeux Met al. Recessive dystrophic epidermolysis bullosa caused by COL7A1 hemizygosity and a missense mutation with complex effects on splicing. Hum Mutat 27:291-2 (2006).Read more (PubMed: 16470588) »
See 1 publication for this product
Publishing research using ab629? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"