TGF beta 2 antibody (ab36495)

Product Name 

TGF beta 2 antibody

Product type 

Primary antibodies

Description 

Mouse monoclonal to TGF beta 2

Immunogen 

An epitope of TGF beta 2 (Human).

Reacts with 
(species key)

Hu, Ms, Cow

Specificity 

Ab36495 recognizes TGF beta 2. It does not recognize TGF beta 1 or TGF beta 3.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/10 - 1/50.
WB: 1/1000. Predicted molecular weight: 50 kDa.

Is unsuitable for Neut.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF

Relevance 

Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.

This antibody does not inhibit the binding of TGF beta 2 to the TGF beta 2 Type 2 Receptor in mink lung epithelial cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for TGF beta 2 antibody images, references, products related to ab36495 and other tools.

TGF beta 2 antibody images:

References for TGF beta 2 antibody (ab36495)

  This product has been used in: (two most recent references)

Bauer M et al. Heterogeneity of gene expression in stromal fibroblasts of human breast carcinomas and normal breast. Oncogene : (2010). IHC-P; Human. PubMed: 20062080

Polak ME et al. Mechanisms of local immunosuppression in cutaneous melanoma. Br J Cancer 96:1879-87 (2007). IHC-P; Human. PubMed: 17565341
If you publish research using ab36495 please let us know so that we can cite the reference on this datasheet.

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