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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta 2 antibody (ab36495)

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Product Name
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TGF beta 2 antibody
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TGF beta 2 抗体 (8件) 一覧
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to TGF beta 2
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Immunogen
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An epitope of TGF beta 2 (Human).
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Reacts with
(species key)
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Hu, Ms, Cow
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Specificity
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Ab36495 recognizes TGF beta 2. It does not recognize TGF beta 1 or TGF beta 3.
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Tested applications
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IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IHC-Fr: 1/10 - 1/50. WB: 1/1000. Predicted molecular weight: 50 kDa.
Is unsuitable for Neut.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors Cancer >> Growth factors >> TGF Stem Cells >> Signaling Pathways >> TGF beta >> Secreted Signal Transduction >> Growth Factors/Hormones >> TGF
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Relevance
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Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.
This antibody does not inhibit the binding of TGF beta 2 to the TGF beta 2 Type 2 Receptor in mink lung epithelial cells.
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TGF beta 2 antibody (ab36495)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None Constituents: Whole serum
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 TGF beta 2 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for TGF beta 2 antibody images, references, products related to ab36495 and other tools.
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TGF beta 2 antibody images:
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta 2 antibody (ab36495)
ab36495 at 1/1000 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was perfomred in citrate buffer. The tissue was then blocked and incubated with the antibody for 1 hour. A biotinylated rabbit anti-mouse antibody was used as the secondary. This image is courtesy of an Abreview submitted by Mr Daniel Bird
See Abreview
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References for TGF beta 2 antibody (ab36495)
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This product has been used in: (two most recent references)
Bauer M et al. Heterogeneity of gene expression in stromal fibroblasts of human breast carcinomas and normal breast. Oncogene : (2010). IHC-P; Human. PubMed: 20062080
Polak ME et al. Mechanisms of local immunosuppression in cutaneous melanoma. Br J Cancer 96:1879-87 (2007). IHC-P; Human. PubMed: 17565341
If you publish research using ab36495 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TGF beta 2
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TGF beta 2 antibody - more information
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Customer reviews (feedback) regarding TGF beta 2 antibody |
Customer FAQs regarding TGF beta 2 antibody |
Protocols for TGF beta 2 antibody |
Price and availability of products related to TGF beta 2 antibody |
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TGF beta 2 antibody - related products:
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TGF beta 2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TGF beta 2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"