Anti-TGF beta 2 antibody (ab36495)

Dear ladies and gentlemen,

I am interested in your TGFb2 antibody (ab36495) and would like to ask you which positive control did you use for westernblot applications? I could not find it in your datasheet.
Thanks in advance.

Kind regards

ANSWER:

Thank you for your inquiry.

This antibody was tested in WB with recombinant TGFb2 which therefore can be used as positive control. We also have a positive Abreview from a customer which used mouse kidney and had veryclear positive staining.

Other positive tissues according to the EST profile of TGFb2are: Thyroid, mammary gland, connective tissue, etc.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

Dear receiver,

We bought antibodies against TGFB2 (ab36495) and TGFB3 (ab53727) to use in WB and we are a bit confused by the only predicted band size given in your datasheet; 50 kDa for TGFB2 and 47 kDa for TGFB3.

1.Are these sizes for the full size pre-pro-TGFB versions?

2.Should one not also detect the C-term mature proteins of around 12-13 kDa as well as possible dimers (approx. 25 kDa)?

3.Do these proteins maybe form tetramers of approx 47-50 kDa?

In the spec sheet for TGFB1 (ab9758) the only predicted bands are the monomer (12 kDa) and the dimer (25 kDa) so the band size info for TGFB2 and -3 was not in line with that and therefore a bit confusing.

Thanks,

ANSWER:

Thank you for your enquiry.

ab9758 - the major band at44.3 kDa corresponding to the unprocessed (pre-pro-TGF beta 1) form, the mature processed TGF beta 1 is a 25 kD homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. As the Western blot image indicates a 25 kDa band is seen in the non-reducing lane and a 12 kDa band in the reducing lane. Please visit UniProt website for further information at: http://www.uniprot.org/uniprot/P01137

ab36495 - the major band at 47.8 kDa corresponding to the unprocessed form;the mature (processed)can form homodimers. UniProt: http://www.uniprot.org/uniprot/P61812

ab53727 - the major band at 47.3 kDacorresponding to the unprocessed form, the mature (processed) can form homodimers. UniProt:http://www.uniprot.org/uniprot/P10600

I hope this helps and if I can assist further, please do not hesitate to contact me.

Dear,

I would like to obtain the material data and safety sheet for Anti-TGF beta 2 antibody (ab36495). I could not find this on your site.

Kind regards,

ANSWER:

Here is the MSDS as requested.


It can also now be accessed from the datasheet of ab36495.


I hope this has been of help. If you have any further questions or requests please do not hesitate to contact us again.

Dear,

I would like to obtain the material data and safety sheet for Anti-TGF beta 2 antibody (ab36495). I could not find this on your site.

Kind regards,

ANSWER:

Thank you for contacting us.


We usually present the MSDS documents to the right hand side of the datasheet of products. The product you are interested in (ab36495) does not have an MSDS presented as it does not contain any materials which can be considered harmful. I have requested that one be generated specifically for you and will forward this to you as soon as I have it.


I hope this information has been on help. If you require any further information, please do not hesitate to ask.

Your TGFB-2 antibody has mouse as host species and the data sheet says it can cross react with mouse. This means that it can recognize TGFb-2 in mouse sample in westren blot. Is this correct? because according to the theory the host species should not be mouse if you want to recognize this protein in mouse samples. Can I use this antody to recognize TGFB-2 in mouse protein samples by western blot. Thanks

ANSWER:

Thank you for your enquiry.
Yes, you are correct that ab36495, although it is a mouse antibody, can recognize mouse TGF beta-2. Here is a review from a customer who tested ab36495 for reactivity to mouse tissue: http://www.abcam.com/index.html?datasheet=36495&tab=abreviews&intabreviewid=8257
Yes, you can use ab26495 to recognize mouse TGF beta 2 via western blotting.
I hope this is helpful. Please contact me again if you have any further questions.