Anti-TGF beta 1 抗体 (ab92486)
Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TGF beta 1 antibody
TGF beta 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TGF beta 1 -
由来種
Rabbit -
特異性
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
ab92486 detects the mature secreted form of the protein at 13 kDa. Our internal testing data suggests that this does not detect the full length protein at 44kDa. As cell lysates are unsuitable for detection of the mature form, tissue lysates should be used when using this antibody.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.
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ポジティブ・コントロール
- WB: Human spleen and kidney tissue lysates.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 5559 Anti-TGF-ß1 Polyclonal Antibody. 5559-100 is the same size as the 100 µg size of ab92486.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), 0.15% EDTA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92486の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa.
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
特記事項 |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
ターゲット情報
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機能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
組織特異性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
関連疾患
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
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別名
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
画像
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All lanes : Anti-TGF beta 1 antibody (ab92486)
Lane 1 : Human recombinant TGF-ß1 at 0.0005 µg
Lane 2 : Human kidney tissue at 50 µg
Lane 3 : Human spleen tissue at 50 µg
Predicted band size: 44 kDa -
All lanes : Anti-TGF beta 1 antibody (ab92486) at 5 µg/ml
Lane 1 : Human spleen tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab92486 observed at 15 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (596)
ab92486 は 596 報の論文で使用されています。
- Yamane S et al. The role of thromboxane prostanoid receptor signaling in gastric ulcer healing. Int J Exp Pathol 103:4-12 (2022). PubMed: 34655121
- Bi L et al. Pirfenidone Attenuates Renal Tubulointerstitial Fibrosis through Inhibiting miR-21. Nephron 146:110-120 (2022). PubMed: 34724669
- Wu J et al. Rubus chingii Hu. unripe fruits extract ameliorates carbon tetrachloride-induced liver fibrosis and improves the associated gut microbiota imbalance. Chin Med 17:56 (2022). PubMed: 35549741
- Mancinelli R et al. The Effects of Taurocholic Acid on Biliary Damage and Liver Fibrosis Are Mediated by Calcitonin-Gene-Related Peptide Signaling. Cells 11:N/A (2022). PubMed: 35563897
- Wang J et al. Empagliflozin Ameliorates Diabetic Cardiomyopathy via Attenuating Oxidative Stress and Improving Mitochondrial Function. Oxid Med Cell Longev 2022:1122494 (2022). PubMed: 35585884