Anti-TGF alpha 抗体 [P/T1] (ab9578)
Key features and details
- Mouse monoclonal [P/T1] to TGF alpha
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-TGF alpha antibody [P/T1]
TGF alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [P/T1] to TGF alpha -
由来種
Mouse -
特異性
This antibody was selected and evaluated immunoserologically for recombinant human TGF-alpha and synthetic (uncoupled) peptides using simple binding and competitive ELISA. Immunohistochemical characterization of the anti-TGF-alpha monoclonal antibody was made on different human carcinomas. Recommended for immunohistochemical detection of TGF-alpha production in human tumor cells, immunoserological monitoring of progression of malignant tumours and for screening of therapeutic efficiency of different human tumors. -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide fragment (32-40 amino acids sequence of 50 amino acids) of transforming growth factor alpha (TGF-alpha).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.097% Sodium azide
Constituents: Tris HCl, 0.2% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
P/T1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
組織特異性
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane and Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 7039 Human
- Omim: 190170 Human
- SwissProt: P01135 Human
- Unigene: 170009 Human
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別名
- EGF like TGF antibody
- EGF-like TGF antibody
- ETGF antibody
see all
画像
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Immunohistochemical characterization of the anti-TGF-a monoclonal antibody ab9578 on human melanoma.
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Overlay histogram showing A431 cells stained with ab9578 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab9578 は 6 報の論文で使用されています。
- Badawy AA et al. Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor Alpha in Cancer Bladder: Schistosomal and Non-Schistosomal. Curr Urol 9:192-201 (2017). PubMed: 28413380
- Schrevel M et al. Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer. Int J Oncol 50:1947-1954 (2017). IHC-P ; Human . PubMed: 28498437
- Mustafi R et al. Both stromal cell and colonocyte epidermal growth factor receptors control HCT116 colon cancer cell growth in tumor xenografts. Carcinogenesis 33:1930-9 (2012). Human . PubMed: 22791816
- Eaton MJ et al. Subarachnoid Transplant of the Human Neuronal hNT2.19 Serotonergic Cell Line Attenuates Behavioral Hypersensitivity without Affecting Motor Dysfunction after Severe Contusive Spinal Cord Injury. Neurol Res Int 2011:891605 (2011). IHC . PubMed: 21799949
- Higgins MJ et al. PIK3CA mutations and EGFR overexpression predict for lithium sensitivity in human breast epithelial cells. Cancer Biol Ther 11:358-67 (2011). PubMed: 21124076
- Bebok Z et al. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use] Orv Hetil 134:1303-7 (1993). PubMed: 8332347