Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (E15 mouse cortex)
Specification
E15 mouse cortex
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 3% BSA and 0.1% Triton X-100
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 546 Goat Anti-Rabbit IgG (H+L) (Molecu
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 546
Dilution
1/1000
Additional data
Additional Notes
Fixed in 4% PFA. No antigen retrieval required.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 01 2013