Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
Yes - tx100
Sample
Human Cell (Human ES cells)
Specification
Human ES cells
Gating Strategy
all cells
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Sample buffer: PBS
Other product details
Incubation time
30 minute(s) · Temperature: 0°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: 647-goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/20000
Additional data
Additional Notes
Lot GR57381-1 worked well for intracellular flow.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 23 2014