Anti-TATA binding protein TBP 抗体 - Loading Control (ab74222)
Key features and details
- Rabbit polyclonal to TATA binding protein TBP - Loading Control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TATA binding protein TBP antibody - Loading Control
TATA binding protein TBP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TATA binding protein TBP - Loading Control -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Snake, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to Human TATA binding protein TBP (N terminal).
Database link: P20226 -
ポジティブ・コントロール
- HeLa or 293T whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74222の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 38 kDa. |
ターゲット情報
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機能
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
組織特異性
Widely expressed, with levels highest in the testis and ovary. -
関連疾患
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
配列類似性
Belongs to the TBP family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395995 Chicken
- Entrez Gene: 431689 Dog
- Entrez Gene: 6908 Human
- Entrez Gene: 21374 Mouse
- Entrez Gene: 117526 Rat
- Omim: 600075 Human
- SwissProt: O13270 Chicken
- SwissProt: P20226 Human
see all -
別名
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
画像
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All lanes : Anti-TATA binding protein TBP antibody - Loading Control (ab74222) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab74222 は 1 報の論文で使用されています。
- Perdigones N et al. Clonal hematopoiesis in patients with dyskeratosis congenita. Am J Hematol 91:1227-1233 (2016). PubMed: 27622320