Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Tissue lysate - nuclear (Liver)
Specification
Liver
Negative control
beads
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA 1%
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA 1%
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%
Additional data
Additional Notes
We have used 5ul of Ab28175 for precipitation with 50ug of chromatin. The enrichment was significantly improved when MNased chromatin was used instead of sonicated.
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投稿 Nov 12 2012