Anti-KDS 抗体 (ab70297)
Key features and details
- Rabbit polyclonal to KDS
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KDS antibody
KDS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KDS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human KDS (C terminal).
Database link: Q9H2K8 -
ポジティブ・コントロール
- WB: HeLa, NIH/3T3 whole cell lysates. IP: HeLa lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab70297 was affinity purified using an epitope specific to JIK immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70297の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
|
IP |
Use at 2-10 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
-
機能
Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK. -
組織特異性
Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 51347 Human
- Entrez Gene: 330177 Mouse
- Entrez Gene: 100174000 Orangutan
- Entrez Gene: 304530 Rat
- Entrez Gene: 446778 Xenopus laevis
- SwissProt: Q9H2K8 Human
- SwissProt: Q9HC79 Human
- SwissProt: Q8BYC6 Mouse
see all -
別名
- CTCL tumor antigen HD CL 09 antibody
- CTCL-associated antigen HD-CL-09 antibody
- Cutaneous T cell lymphoma tumor antigen HD CL 09 antibody
see all
画像
-
Immunoprecipitation of HeLa whole cell lysate using ab70297.
Lane 1: ab70297 current lot
Lane 2: ab70297 previous lot
Lane 3: other rabbit anti-KDS antibody
Lane 4: Control IgG
-
All lanes : Anti-KDS antibody (ab70297) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 105 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab70297 は 2 報の論文で使用されています。
- Ormonde JVS et al. TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK. J Immunol 201:3431-3442 (2018). PubMed: 30373850
- Hammad H et al. Transitional B cells commit to marginal zone B cell fate by Taok3-mediated surface expression of ADAM10. Nat Immunol 18:313-320 (2017). PubMed: 28068307