Application
Western blot
Sample
Human Cell lysate - whole cell (SW780 bladder cancer)
Loading amount
25 µg
Specification
SW780 bladder cancer
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG P0447 Dako Cytomation HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 40 and 90 kDa
Additional data
Additional Notes
lane 1) All Blue Biorad 2) 1:1250 3)1:2500, 4) 1:5000, 5) 1:10.000
bands at 40kDa (predicted) and two additional high molecular weight bands
bands at 40kDa (predicted) and two additional high molecular weight bands
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Karin Birkenkamp-Demtroeder
Verified customer
投稿 Apr 30 2010