Application
Western blot
Sample
Human Cell lysate - whole cell (Human A549 epithelial aveolar basal adenocarcinoma)
Loading amount
10 µg
Specification
Human A549 epithelial aveolar basal adenocarcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (whole molecule)–Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 22 2011