Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
3% BSA and 10% normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Rhesus monkey Tissue sections (colon)
Specification
colon
Permeabilization
Yes - 0.1% Triton-X-100
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10 mM Tris-HCl pH 7.5 150 mM NaCl, 3% BS
Dilution
1/10
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG1 Alexa 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Antigen retrieval was also done using high pH Tris-EDTA buffer in addition to citrate buffer. Primary antibody incubation was also done overnight at 4 C.
Abcam response
We thank this reviewer for testing this antibody in macaque through the abTrial program. To our knowledge, this had not been done before, and the reviewer received a discount code worth the full amount of the antibody for testing. Information regarding the abTrial program is given through the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=11998
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 30 2013