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- Secondary antibodies
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Product name
Anti-Synaptophysin antibody
Synaptophysin 抗体 (24件) 一覧
Description
Rabbit polyclonal to Synaptophysin
Tested applications
WB, IHC-P, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide from rat or mouse Synaptophysin conjugated to an immunogenic carrier protein.
Positive control
1 day- old mouse cortical cells, rat trigeminal nerve tissue, rat brain lysate.
Properties
Form
Lyophilised:
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> SNAPs & SNAREs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/2000.
IHC-P: 1/300 - 1/3000. Perform antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Tissue specificity
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Involvement in disease
Defects in SYP are the cause of mental retardation X-linked SYP-related (MRXSYP) [MIM:300802]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
Sequence similarities
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain.
Domain
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
Post-translational
modifications
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
Target information above from: UniProt accessionP08247
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Major synaptic vesicle protein P38 antibody
- Syn p38 antibody
- Synaptophysin antibody
see all
Database links
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- SwissProt: Q62277 Mouse
- SwissProt: P07825 Rat
- Unigene: 223674 Mouse
- Unigene: 11067 Rat
Anti-Synaptophysin antibody images:
Western blot - Synaptophysin antibody (ab68851)
All lanes : Anti-Synaptophysin antibody (ab68851) at 1/1000 dilution
Lane 1 : Rat brain lysate, untreated
Lane 2 : Rat brain lysate, cells pretreated with the immunising peptide
Predicted band size : 33 kDa
Observed band size : 33 kDa
Additional bands at : >75 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Synaptophysin antibody (ab68851)
1 day old mouse cortical cell culture stained with ab68851 at 1/2000 dilution. DAPI counter stained.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptophysin antibody (ab68851)
Rat trigeminal nerve tissue stained with ab68851 at 1/2000 dilution.
References for Anti-Synaptophysin antibody (ab68851)
This product has been referenced in:
- Micheva KD et al. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron 68:639-53 (2010). Mouse.Read more (PubMed: 21092855) »
See 1 publication for this product
Publishing research using ab68851? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"