Application
Immunoprecipitation
Sample
Human Cell lysate - other (HeLa)
Total protein in input
25 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Other product details
Dilution
1/1000
Incubation time
40 minute(s)
Western Blot antibody
Name
Non-Abcam antibody was used: Anti rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
HeLa cells clear lysate (CL) as well as the bound material (B) were loaded on a 15 % acrylamid gel.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sandrine Ruchaud
Verified customer
投稿 Apr 12 2006