Application
Western blot
Sample
Rabbit Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Loading amount
25 µg
Specification
lung
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-mouse IgG-HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Positive control
actin
Additional data
Additional Notes
Foxm1 observed rabbit lung.
Foxm1 also observed human antibody.
an0ther band is 70KDa. I think 70KDa band something.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Mun-Suk Park
Verified customer
投稿 Nov 01 2011