Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE gel)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Tris-Salin-Tween 20 (TST) buffer
Dilution
1/1500
Secondary antibody
Name
Non-Abcam antibody was used: Anti rabbit IgG-HRP antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Thermo Scientific SuperSignal West Pico
Bands
Specific: 15, 17 kDa kDa
Exposure
15 second(s)
Additional data
Additional Notes
Got some background in this blot but it can be get rid off by extended washings.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 07 2014