Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblast)
Loading amount
20 µg
Specification
Human fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris nupage)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa Non-specific: 14 kDa
Additional data
Additional Notes
Bands ranging from 50kD-160kD might be sumolyated target proteins, With MG132, these bands intensity increased.
14 kd band might be the shorter variant of Sumo2
14 kd band might be the shorter variant of Sumo2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 22 2012