Application
Western blot
Sample
Pig Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
RPE
Blocking step
Milk (left) and BSA (right) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 17 kDa
Additional data
Additional Notes
Band at 17 kDa is non conjugated SUMO.
More bands observed with BSA as a blocking agent.
Not determined it they are specific or not as SUMOylated proteins can occur at any sizes.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 22 2018