Application
Western blot
Sample
Human Recombinant protein (Sumoylation assay of P.O.I.)
Loading amount
0.0125 µg
Specification
Sumoylation assay of P.O.I.
Treatment
Sumoylation Assay 3hrs.
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce West Pico
Exposure
30 second(s)
Bands
Specific: 16, 75 kDa
Positive control
P53 is the pos control. 16kDa is the free Sumo band and 75 kDa is my sumoylated protein.
Negative control
I use a mutant sumo protein in the sumoylation assays as the negative control. It does not react with this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Don Anderson
Verified customer
投稿 Aug 23 2006