Anti-Strep-tag II 抗体 (ab76950)
Key features and details
- Rabbit polyclonal to Strep-tag II
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Strep-tag II antibody
Strep-tag II 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Strep-tag II -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Amino acid sequence gggWSHPQFEK.
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ポジティブ・コントロール
- Streptavidin tagged recombinant protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity chromatography purified via peptide column -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76950の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa. |
ターゲット情報
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関連性
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
別名
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
画像
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All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml
Lane 1 : Streptavidin tagged recombinant protein with blocking peptide
Lane 2 : Streptavidin tagged recombinant protein
Lysates/proteins at 0.05 µg per lane.
Predicted band size: 24 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab76950 は 4 報の論文で使用されています。
- Reichmann J et al. Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. J Cell Biol 219:N/A (2020). PubMed: 32232464
- Long T et al. Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition. Nat Commun 10:2452 (2019). PubMed: 31165728
- Baggett NE et al. Global analysis of translation termination in E. coli. PLoS Genet 13:e1006676 (2017). PubMed: 28301469
- Lin Y et al. Antiaging Gene Klotho Deficiency Promoted High-Fat Diet-Induced Arterial Stiffening via Inactivation of AMP-Activated Protein Kinase. Hypertension 67:564-73 (2016). PubMed: 26781278