Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult testis)
Specification
adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500
Additional data
Additional Notes
Although the Stra8 can be detected, the fluorescent image was rather weak. Maybe it can be increased by increasing the primary antibody concentration.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 煜欽 林
Verified customer
投稿 May 17 2013