Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Permeabilization
No
Specification
embryonic stem cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 16°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: block
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
DAPI staining used to show cell nucleus. Cells were fixed in 4% PFA and were not permeabilised. I found that use of triton X or methanol/acetone fixation gave much less clear staining than PFA without triton X.
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投稿 Oct 11 2012