Anti-Cystatin-B 抗体 [2F1] (ab72353)
Key features and details
- Mouse monoclonal [2F1] to Cystatin-B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Cystatin-B antibody [2F1]
Cystatin-B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2F1] to Cystatin-B -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Cystatin-B.
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ポジティブ・コントロール
- A549 cell lysate
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特記事項
This product was changed from ascites to tissue culture supernatant on 08/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2F1 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
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ICC/IF |
1/100.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa). |
ICC/IF
1/100. |
ターゲット情報
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機能
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. -
関連疾患
Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. -
配列類似性
Belongs to the cystatin family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1476 Human
- Omim: 601145 Human
- SwissProt: P04080 Human
- Unigene: 695 Human
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別名
- CHROW21 antibody
- CPI B antibody
- CPI-B antibody
see all
画像
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ICC/IF analysis of CSTB in A549 cells. The cell was stained with ab72353(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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All lanes : Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : U87MG cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 11 kDa -
Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab72353 は 1 報の論文で使用されています。
- Burgener A et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . PubMed: 23449785