Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Human Tissue sections (skin (from atopic patient))
Specification
skin (from atopic patient)
Permeabilization
Yes - 0,1% Saponin / PBS
Fixative
Acetone
Other product details
Concentration
10 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Concentration
15 µg/ml
Name
Non-Abcam antibody was used: goat a-rabbit Staph. aureus Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 17 2013