Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (8-cell stage mouse pre-implantation embryo)
Permeabilization
Yes - 0.5% Triton X-100 in 1xPBS
Specification
8-cell stage mouse pre-implantation embryo
Blocking step
BSA as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
The image of an 8-cell stage pre-implantation mouse embryo that was injected with wtSpry4 (C-terminally tagged with eGFP) mRNA at 2-cell stage (in green) and stained with anti-Spry4 antibody (ab59785) (in red). Nuclei are stained with DAPI (in blue).
Abcam response
Thank you for your valuable feedback. This antibody has not been tested in ICC/IF before and therefore may not be suitable for this application. We welcome other abreviews to confirm this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Aleksandar Mihajlovic
Verified customer
投稿 Sep 16 2013