Anti-Spermine 抗体 (ab26975)
Key features and details
- Rabbit polyclonal to Spermine
- Suitable for: ICC/IF, WB
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-Spermine antibody -
製品の詳細
Rabbit polyclonal to Spermine -
由来種
Rabbit -
特異性
This antibody reacts with spermine. Cross-reacts with spermidine 100%. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Species independent -
免疫原
Spermine conjugated to KLH.
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特記事項
Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26975の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB |
1/1200 - 1/5000.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1200 - 1/5000. |
ターゲット情報
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関連性
Spermine, a polyamine, is a positively charged compound found in living cells and can cause conformational changes in DNA via charge and solubility mechanisms which can affect gene expression cell differentiation. Spermine may be linked to the development of cancer. -
別名
- Spermine antibody
画像
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ab26975 staining spermine in the Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehyde for 15 minuts at room temperature (Upper images) or iced methanol of 3 minutes (Lower images) and blocked with 2% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/100) for 1 hour at 27°C. An Alexa Fluor®568-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. Samples handled without ab26975 were used as negative controls (Left-hand panels).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab26975 は 5 報の論文で使用されています。
- Vila A et al. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Front Cell Neurosci 15:667046 (2021). PubMed: 34393723
- Do THT et al. Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes. Proc Natl Acad Sci U S A 116:12540-12549 (2019). PubMed: 31152136
- Maki K et al. Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish. J Biol Chem 292:6369-6380 (2017). WB . PubMed: 28258224
- Nakajima K et al. KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis. Nat Commun 6:8532 (2015). PubMed: 26449415
- Gaur V et al. Crystal structure and functional insights of hemopexin fold protein from grass pea. Plant Physiol 152:1842-50 (2010). PubMed: 20147493